Push-pull perfusion sampling with segmented flow for high temporal and spatial resolution in vivo chemical monitoring.

@article{Slaney2011PushpullPS,
  title={Push-pull perfusion sampling with segmented flow for high temporal and spatial resolution in vivo chemical monitoring.},
  author={Thomas R Slaney and Jing Nie and Neil D Hershey and Prasanna K Thwar and Jennifer J. Linderman and Mark A. Burns and Robert T Kennedy},
  journal={Analytical chemistry},
  year={2011},
  volume={83 13},
  pages={5207-13}
}
Low-flow push-pull perfusion is a sampling method that yields better spatial resolution than competitive methods like microdialysis. Because of the low flow rates used (50 nL/min), it is challenging to use this technique at high temporal resolution which requires methods of collecting, manipulating, and analyzing nanoliter samples. High temporal resolution also requires control of Taylor dispersion during sampling. To meet these challenges, push-pull perfusion was coupled with segmented flow to… CONTINUE READING