Puromycin-based purification of rat brain capillary endothelial cell cultures. Effect on the expression of blood-brain barrier-specific properties.

@article{Perrire2005PuromycinbasedPO,
  title={Puromycin-based purification of rat brain capillary endothelial cell cultures. Effect on the expression of blood-brain barrier-specific properties.},
  author={Nicolas Perri{\`e}re and Ph Demeuse and Esteban Omar Garcia and Anthony R{\'e}gina and Marcel Debray and J P Andreux and Patrick Couvreur and J-M Scherrmann and Jamal Temsamani and P-O Couraud and M{\'a}ria A Deli and Françoise Roux},
  journal={Journal of neurochemistry},
  year={2005},
  volume={93 2},
  pages={279-89}
}
One of the main difficulties with primary rat brain endothelial cell (RBEC) cultures is obtaining pure cultures. The variation in purity limits the achievement of in vitro models of the rat blood-brain barrier. As P-glycoprotein expression is known to be much higher in RBECs than in any contaminating cells, we have tested the effect of five P-glycoprotein substrates (vincristine, vinblastine, colchicine, puromycin and doxorubicin) on RBEC cultures, assuming that RBECs would resist the treatment… CONTINUE READING
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