Puromycin Selection Confounds the RNA-Seq Profiles of Primary Human

  title={Puromycin Selection Confounds the RNA-Seq Profiles of Primary Human},
  author={Shuqing Guo},
Lentiviral transduction followed by puromycin selection is a well-recognized procedure for gene transfer and expression experiments using a variety of cell types including human hematopoietic stem and progenitor cells. Despite its widespread application, research regarding the potential effects of bacterial puromycin N-acetyltransferase (pac) gene expression in mammalian cell cultures is incomplete. Here the potential for puromycin selection to affect transcriptome profiles was examined using a… CONTINUE READING

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