Purity, cell viability, expression of GFAP and bystin in astrocytes cultured by different procedures.

@article{Du2010PurityCV,
  title={Purity, cell viability, expression of GFAP and bystin in astrocytes cultured by different procedures.},
  author={Fang Du and Zhong-Ming Qian and Li Zhu and Xiao Mei Wu and Christopher Qian and Robbie Yat-Kan Chan and Ya Ke},
  journal={Journal of cellular biochemistry},
  year={2010},
  volume={109 1},
  pages={30-7}
}
Primary astrocyte cultures are the most commonly used in vitro model for neurobiological studies. We speculated that different protocols might induce differences not only in the percentage of astrocytes but also in their biological characteristics. In this study, we investigated the effects of four major protocols on the purity of astrocytes, cell viability, expression of glial fibrillary acidic protein (GFAP) and bystin of cultured astrocytes using MTT assay, immunocytochemical staining, and… CONTINUE READING
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