Purine nucleoside phosphorylase. 2. Catalytic mechanism.

@article{Erion1997PurineNP,
  title={Purine nucleoside phosphorylase. 2. Catalytic mechanism.},
  author={M. Erion and J. Stoeckler and W. Guida and R. Walter and S. Ealick},
  journal={Biochemistry},
  year={1997},
  volume={36 39},
  pages={
          11735-48
        }
}
X-ray crystallography, molecular modeling, and site-directed mutagenesis were used to delineate the catalytic mechanism of purine nucleoside phosphorylase (PNP). PNP catalyzes the reversible phosphorolysis of purine nucleosides to the corresponding purine base and ribose 1-phosphate using a substrate-assisted catalytic mechanism. The proposed transition state (TS) features an oxocarbenium ion that is stabilized by the cosubstrate phosphate dianion which itself functions as part of a catalytic… Expand
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References

Gaussian Inc., Pittsburgh, PA
  • 1993