Purine nucleoside phosphorylase from human erythrocytes.

@article{Stoeckler1978PurineNP,
  title={Purine nucleoside phosphorylase from human erythrocytes.},
  author={J. Stoeckler and R. Agarwal and K. Agarwal and R. Parks},
  journal={Methods in enzymology},
  year={1978},
  volume={51},
  pages={
          530-8
        }
}
Purine nucleoside phosphorylase has been purified about 7,300-fold and crystallized from human erythrocytes (mol wt 81,000). The recrystallized enzyme exists in the form of needles and sometimes bundles of needles and has a specific activity of 96 pM units per mg of protein. A number of phenomena reported earlier for a less pure preparation of this enzyme are still seen with the crystalline enzyme. These include (a) substrate activation by inosine and (b) a constant ratio of reactivity with… Expand
Characterization of the subunits of purine nucleoside phosphorylase from cultured normal human fibroblasts
Overexpression, purification and characterization of functional calf purine nucleoside phosphorylase (PNP).
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References

DEVERDIER, k.-H., AND G&L;, B
  • j., Biochim. Biophys. Acta.,
  • 1964