Purine nucleobase transport in human erythrocytes. Reinvestigation with a novel "inhibitor-stop" assay.

@article{Domin1988PurineNT,
  title={Purine nucleobase transport in human erythrocytes. Reinvestigation with a novel "inhibitor-stop" assay.},
  author={Barbara A. Domin and William B Mahony and Thomas P Zimmerman},
  journal={The Journal of biological chemistry},
  year={1988},
  volume={263 19},
  pages={9276-84}
}
A novel "inhibitor-stop" method for the determination of initial rates of purine nucleobase transport in human erythrocytes has been developed, based on the addition of seven assay volumes of cold 19 mM papaverine to terminate influx. In view of our finding that the initial velocities of adenine, guanine, and hypoxanthine influx into human erythrocytes were linear for only 4-6 s at 37 degrees C, the present method has been used to reexamine the kinetics of purine nucleobase transport in these… CONTINUE READING