Purified tomato polygalacturonase activity during thermal and high-pressure treatment.

  title={Purified tomato polygalacturonase activity during thermal and high-pressure treatment.},
  author={Isabel Verlent and Ann M van Loey and Chantal Smout and Thomas Duvetter and Marc E G Hendrickx},
  journal={Biotechnology and bioengineering},
  volume={86 1},
Extracted tomato polygalacturonase was purified by cation-exchange chromatography (and gel filtration) and characterized for molar mass, isoelectric point, as well as optimal pH for polygalacturonase activity. The enzymatic reaction of purified tomato polygalacturonase on polygalacturonic acid as substrate was investigated during a combined high-pressure/temperature treatment in a temperature range of 25 degrees to 80 degrees C and in a pressure range of 0.1 to 500 MPa at pH 4.4 (the pH of… CONTINUE READING
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