Purified recombinant 60 kD SS-A/Ro protein--an antigenic source for ELISA screening of patients with rheumatic diseases.

Abstract

A 60 kD SS-A/Ro recombinant protein was expressed in E. coli cells and purified by the centrifugation procedure. Since the substrate contained a substantial amount of bacterial proteins it was not sufficient for ELISA. Therefore the material obtained by centrifugation was resolved on SDS-gel and the 60 kD region was excised and electro-eluted. Two hundred… (More)

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