Purified presequence binding factor (PBF) forms an import-competent complex with a purified mitochondrial precursor protein.

@article{Murakami1990PurifiedPB,
  title={Purified presequence binding factor (PBF) forms an import-competent complex with a purified mitochondrial precursor protein.},
  author={Kazushi Murakami and Masataka Mori},
  journal={The EMBO journal},
  year={1990},
  volume={9 10},
  pages={3201-8}
}
In vitro mitochondrial import of the purified precursor form (pOTC) of rat ornithine carbamoyltransferase (OTC) is stimulated by a cytosolic factor(s) contained in rabbit reticulocyte lysate. A protein factor that binds to pOTC but not to mature OTC and was named presequence binding factor or PBF, was purified 91,000-fold from the lysate by affinity chromatography using pOTC-bound Sepharose, DEAE-5PW HPLC and sucrose gradient centrifugation. The purified PBF migrated as a single polypeptide of… CONTINUE READING