Purified phi 6 nucleocapsids are capable of productive infection of host cells with partially disrupted outer membranes.

@article{Ojala1990PurifiedP6,
  title={Purified phi 6 nucleocapsids are capable of productive infection of host cells with partially disrupted outer membranes.},
  author={P{\"a}ivi M Ojala and Martin Romantschuk and Dennis Henry Bamford},
  journal={Virology},
  year={1990},
  volume={178 2},
  pages={364-72}
}
Purified nucleocapsids of bacteriophage phi 6, lacking the phage lipid envelope, are unable to infect intact Pseudomonas syringae host cells. A method for studying the process by which a naked virus particle, the phi 6 nucleocapsid, penetrates the host cytoplasmic membrane was developed. Host cells were rendered competent for nucleocapsid infection by treatment with repeated washings with salt and sucrose and the subsequent addition of lysozyme. This treatment disrupts the outer membrane… CONTINUE READING

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