Purified Ribulose-P2 carboxylase from wheat with high specific activity and with fast activation

Abstract

Isolation of Ribulose-P2 carboxylase/oxygenase and its purification to homogeneity often results in losses of enzyme activity. Attempts were made to decrease these losses by adding fructose-P2, SO ] , Pi or PPi to the buffers used during early stages of the extraction and purification of the enzyme. Yield and final specific activity were not improved, but… (More)
DOI: 10.1007/BF00018374

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