Purified Radiolabeled Antithrombin III Metabolism in Three Families With Hereditary AT Ill Deficiency: Application of a Three-Compartment Model

Abstract

Purified human radioiodinated antithrombin Ill (‘251-AT Ill) was used to study its metabolism in six members from three different families with a known hereditary AT Ill deficiency. Six healthy volunteers served as a control group. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and crossed immunoelectrophoresis (CIE) showed the purified AT Ill to be homogenous. Amino acid analysis of the protein revealed a composition identical to a highly purified internal standard. The specific activity was 5.6 U/mg. Analysis of plasma radioactivity data was performed, using a three-compartment model. Neither plasma disappearance half-times nor fractional

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Cite this paper

@inproceedings{Knot2005PurifiedRA, title={Purified Radiolabeled Antithrombin III Metabolism in Three Families With Hereditary AT Ill Deficiency: Application of a Three-Compartment Model}, author={E . A . R . Knot and Edwin de Jong and Jan Wouter ten Cate and A . H . C . lburg and Ch Pieter Henny and Taco Bruin and Jeanne Stibbe}, year={2005} }