Purification to homogeneity of pig leucocyte catabolin, a protein that causes cartilage resorption in vitro.

Abstract

Catabolin, a protein that causes proteoglycan resorption in explants of living cartilage, was purified to homogeneity from culture medium conditioned by culturing buffy-coat leucocytes from 60 litres of pig blood in the presence of concanavalin A. The purification steps were (1) gel filtration of concentrated medium, (2) chromatofocusing, (3) hydroxyapatite… (More)

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@article{Saklatvala1983PurificationTH, title={Purification to homogeneity of pig leucocyte catabolin, a protein that causes cartilage resorption in vitro.}, author={Jeremy Saklatvala and Valerie A. Curry and Simon J Sarsfield}, journal={The Biochemical journal}, year={1983}, volume={215 2}, pages={385-92} }