Purification of unactivated progesterone receptor and identification of novel receptor-associated proteins.

@article{Smith1990PurificationOU,
  title={Purification of unactivated progesterone receptor and identification of novel receptor-associated proteins.},
  author={David F. Smith and Lee E. Faber and David O. Toft},
  journal={The Journal of biological chemistry},
  year={1990},
  volume={265 7},
  pages={3996-4003}
}
Progesterone receptor complexes were purified from crude cytosol in a rapid, gentle, one-step procedure using anti-receptor monoclonal antibodies covalently attached to an agarose resin. Five nonreceptor proteins specifically co-purified with unactivated avian progesterone receptor; these proteins had molecular masses of approximately 90, 70, 54, 50, and 23 kDa. The 90- and 70-kDa proteins have been previously identified as the 90-kDa heat shock protein and a member of the 70-kDa heat shock… CONTINUE READING