Purification of two types of gonadotropin receptors from carp ovarian follicles: overlapping recognition by two different ligands.

@article{Basu2002PurificationOT,
  title={Purification of two types of gonadotropin receptors from carp ovarian follicles: overlapping recognition by two different ligands.},
  author={Dipanjan Basu and Samir Bhattacharya},
  journal={General and comparative endocrinology},
  year={2002},
  volume={129 3},
  pages={152-62}
}
Teleostean gonadotropin receptors were solubilized from the plasma membrane preparation of carp ovarian follicles by lithium diiodosalicylate and Triton X-100. Solubilization resulted fourfold increase in GTH binding activity as compared to the crude plasma membrane preparation. An addition of 25% glycerol and protease inhibitors to the solubilized receptor retained more than 90% original activity at -20 degrees C for 30 days. Gel filtration through Sephadex G 100 significantly increased the… CONTINUE READING

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