Purification of two plaque variants of Sindbis virus

@article{Bose2005PurificationOT,
  title={Purification of two plaque variants of Sindbis virus},
  author={Henry R. Bose, and G. Z. Carl and Bernard P. Sagik},
  journal={Archiv f{\"u}r die gesamte Virusforschung},
  year={2005},
  volume={31},
  pages={200-206}
}
A method for concentrating and purifying enveloped viruses is described. Its salient feature is the use of an antihost immunoadsorbent column. Purified virus preparations were obtained in which viral-specific proteins per/mg total protein were enriched 800-fold, while host-specific complement fixing material became undetectable. 
2 Citations
Characterization of a Sindbis virus variant with altered host range
TLDR
Both viral glycoproteins E1 and E2 from the variant were more negatively charged than those of the standard virus but the authors were unable to find changes in tryptic peptides of the variant. Expand
Peptide composition of Sindbis virus variants
TLDR
The peptide composition of variants of Sindbis virus which differ in plaque-type, elution pattern from calcium phosphate columns and virulence in mice was analyzed and the hemagglutinating components obtained from these plaque- type variants eluted from the column at the same phosphate molarity. Expand

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