Purification of two lipovitellins and development of immunoassays for two forms of their precursors (vitellogenins) in medaka (Oryzias latipes).

@article{Fujiwara2005PurificationOT,
  title={Purification of two lipovitellins and development of immunoassays for two forms of their precursors (vitellogenins) in medaka (Oryzias latipes).},
  author={Yumi Fujiwara and Haruhisa Fukada and Munetaka Shimizu and Akihiko Hara},
  journal={General and comparative endocrinology},
  year={2005},
  volume={143 3},
  pages={267-77}
}
Two distinct yolk proteins (YP1 and YP2) were purified from the ovary of medaka, and specific antisera against YPs were generated to characterize YPs and reveal their relation to two vitellogenins (Vg1 and Vg2). The molecular masses of purified YP1 and YP2 on gel filtration were 270 and 380 kDa, respectively. YPs were confirmed to be lipoproteins by staining with Sudan black. Amino acid compositions of YP1 and YP2 were similar to those of Vg1 and Vg2, respectively. In double immunodiffusion… CONTINUE READING
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