Purification of transmembrane proteins from Saccharomyces cerevisiae for X-ray crystallography.

Abstract

To enhance the quantity and quality of eukaryotic transmembrane proteins (TMPs) available for structure determination by X-ray crystallography, we have optimized protocols for purification of TMPs expressed in the yeast Saccharomyces cerevisiae. We focused on a set of the highest-expressing endogenous yeast TMPs for which there are established biochemical… (More)
DOI: 10.1016/j.pep.2009.12.012

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@article{Clark2010PurificationOT, title={Purification of transmembrane proteins from Saccharomyces cerevisiae for X-ray crystallography.}, author={Kathleen M Clark and Nadia Fedoriw and Katrina Robinson and Sara M Connelly and J. W. Randles and Michael G. Malkowski and George T. DeTitta and Mark E Dumont}, journal={Protein expression and purification}, year={2010}, volume={71 2}, pages={207-23} }