Purification of thymidylate synthetase from enzyme-poor sources by affinity chromatography.

@article{Slavik1976PurificationOT,
  title={Purification of thymidylate synthetase from enzyme-poor sources by affinity chromatography.},
  author={K J Slavik and Wojciech Rode and V{\'e}ra Slav{\'i}kov{\'a}},
  journal={Methods in enzymology},
  year={1976},
  volume={66},
  pages={709-23}
}
The adsorption of thymidylate synthetase from Escherichia coli B to aminoalkyl-Sepharose with the increasing length of carbon chain (2--6 carbon atoms) was investigated. A correlation was found between the chain length and adsorption effectiveness, increasing from the two- to the six-carbon chain. A hydrophobic chromatography of the enzyme on aminobutyl… CONTINUE READING