Purification of the three nuclear RNA polymerases from Neurospora crassa.

@article{Armaleo1985PurificationOT,
  title={Purification of the three nuclear RNA polymerases from Neurospora crassa.},
  author={Daniele Armaleo and S. R. Gro\ss},
  journal={The Journal of biological chemistry},
  year={1985},
  volume={260 30},
  pages={16169-73}
}
Nuclear RNA polymerases I, II, and III from Neurospora crassa have been purified 3,000-, 1,500-, and 10,000-fold, respectively, by a procedure that minimizes proteolysis of the 220-kDa subunit of polymerase II. The Neurospora enzymes resemble, in polypeptide composition, the corresponding polymerases isolated from other eukaryotes. The 220-kDa subunit of… CONTINUE READING