Purification of the simian virus 40 (SV40) T antigen DNA-binding domain and characterization of its interactions with the SV40 origin.

@article{Joo1997PurificationOT,
  title={Purification of the simian virus 40 (SV40) T antigen DNA-binding domain and characterization of its interactions with the SV40 origin.},
  author={W. S. Joo and X. Luo and D. Denis and H. Kim and G. Rainey and C. Jones and K. R. Sreekumar and P. Bullock},
  journal={Journal of virology},
  year={1997},
  volume={71 5},
  pages={
          3972-85
        }
}
  • W. S. Joo, X. Luo, +5 authors P. Bullock
  • Published 1997
  • Medicine, Biology
  • Journal of virology
  • To better define protein-DNA interactions at a eukaryotic origin, the domain of simian virus 40 (SV40) large T antigen that specifically interacts with the SV40 origin has been purified and its binding to DNA has been characterized. Evidence is presented that the affinity of the purified T antigen DNA-binding domain for the SV40 origin is comparable to that of the full-length T antigen. Furthermore, stable binding of the T antigen DNA-binding domain to the SV40 origin requires pairs of… CONTINUE READING

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