Purification of the simian virus 40 (SV40) T antigen DNA-binding domain and characterization of its interactions with the SV40 origin.

@article{Joo1997PurificationOT,
  title={Purification of the simian virus 40 (SV40) T antigen DNA-binding domain and characterization of its interactions with the SV40 origin.},
  author={Woo S Joo and Xiaomin Luo and David J. Denis and H Y Kim and G. Jonah A. Rainey and Carol Jones and Kodangattil R Sreekumar and Peter A. Bullock},
  journal={Journal of virology},
  year={1997},
  volume={71 5},
  pages={3972-85}
}
To better define protein-DNA interactions at a eukaryotic origin, the domain of simian virus 40 (SV40) large T antigen that specifically interacts with the SV40 origin has been purified and its binding to DNA has been characterized. Evidence is presented that the affinity of the purified T antigen DNA-binding domain for the SV40 origin is comparable to that of the full-length T antigen. Furthermore, stable binding of the T antigen DNA-binding domain to the SV40 origin requires pairs of… CONTINUE READING
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Structure of the origin specific DNA binding domain from simian virus 40 T-antigen

  • X. Luo, D. G. Sanford, P. A. Bullock, W. W. Bachovchin
  • Nature Struct. Biol
  • 1996
Highly Influential
4 Excerpts

Molecular cloning: a laboratory manual, 2nd ed

  • J. Sambrook, E. F. Fritsch, T. Maniatis
  • 1989
Highly Influential
6 Excerpts

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