Purification of the rat-liver mitochondrial dicarboxylate carrier by affinity chromatography on immobilized malate dehydrogenase.

@article{LanarBenba1994PurificationOT,
  title={Purification of the rat-liver mitochondrial dicarboxylate carrier by affinity chromatography on immobilized malate dehydrogenase.},
  author={J Lançar-Benba and Barbara Foucher and M Saint-Macary},
  journal={Biochimica et biophysica acta},
  year={1994},
  volume={1190 2},
  pages={213-6}
}
The dicarboxylate carrier of rat-liver mitochondria, extracted by Triton X-100 and partially purified by hydroxylapatite chromatography, was retained by malate dehydrogenase immobilized on Sepharose gel, and eluted with 0.4 M NaCl. SDS-polyacrylamide gel electrophoresis of the eluate showed a predominant peptide band with an M(r) of 28,000. The purified protein, incorporated into liposomes, mediated a butylmalonate sensitive malonate(out)/malate(in) exchange that was inhibited by p… CONTINUE READING