Purification of the major endoglucanase from Aspergillus fumigatus Fresenius.

@article{Parry1983PurificationOT,
  title={Purification of the major endoglucanase from Aspergillus fumigatus Fresenius.},
  author={Janet Parry and James C. Stewart and J Heptinstall},
  journal={The Biochemical journal},
  year={1983},
  volume={213 2},
  pages={437-44}
}
Aspergillus fumigatus (Fresenius), IMI 246651, A.T.C.C. 46324, produces two beta-glucosidase enzymes, cotton-solubilizing activity, xylanase and endoglucanase enzymes which can be separated by gel-filtration chromatography. The major endoglucanase does not bind to concanavalin A-Sepharose and does not stain with periodic acid/Schiff reagent. It is homogeneous on polyacrylamide isoelectric focusing (pI = 7.1) and has a mol.wt. of 12500 by sodium dodecyl sulphate/polyacrylamide-gel… CONTINUE READING