Purification of the beta-cell glucose-sensitive factor that transactivates the insulin gene differentially in normal and transformed islet cells.

@article{Marshak1996PurificationOT,
  title={Purification of the beta-cell glucose-sensitive factor that transactivates the insulin gene differentially in normal and transformed islet cells.},
  author={Sonya Marshak and Hanan Totary and Erol Cerasi and Danielle Melloul},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1996},
  volume={93 26},
  pages={15057-62}
}
The beta cell-specific glucose-sensitive factor (GSF), which binds the A3 motif of the rat I and human insulin promoters, is modulated by extracellular glucose. A single mutation in the GSF binding site of the human insulin promoter abolishes the stimulation by high glucose only in normal islets, supporting the suggested physiological role of GSF in the glucose-regulated expression of the insulin gene. GSF binding activity was observed in all insulin-producing cells. We have therefore purified… CONTINUE READING
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