Purification of secreted α-amylases by immunoaffinity chromatography with cross-reactive antibody

@article{Katoh1994PurificationOS,
  title={Purification of secreted α-amylases by immunoaffinity chromatography with cross-reactive antibody},
  author={Shigeo Katoh and Masaaki Terashima},
  journal={Applied Microbiology and Biotechnology},
  year={1994},
  volume={42},
  pages={36-39}
}
Two isozymes of rice α-amylases expressed and secreted by recombinant yeast were purified by immunoaffinity chromatography by using cross-reactive antibody. Antibodies raised against partially purified barley α-amylase adsorbed rice α-amylases in fermentation broth by a cross-reaction. By use of these antibodies as ligands, rice α-amylases were concentrated and purified to a high degree in one-step immunoaffinity chromatography. Because of the differences in the contaminating impurities between… CONTINUE READING
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