Purification of recombinant ribulose-1,5-bisphosphate carboxylase/oxygenase large subunits suitable for reconstitution and assembly of active L8S8 enzyme.

@article{Lee1990PurificationOR,
  title={Purification of recombinant ribulose-1,5-bisphosphate carboxylase/oxygenase large subunits suitable for reconstitution and assembly of active L8S8 enzyme.},
  author={Bo Gyeong Lee and F Robert Tabita},
  journal={Biochemistry},
  year={1990},
  volume={29 40},
  pages={9352-7}
}
Ribulose-1,5-bisphosphate carboxylase/oxygenase (RubisCO) from Anacystis nidulans was reconstituted in vitro from extracts of Escherichia coli strains that separately express large and small subunits. This reconstitution system was shown to be useful for monitoring the appearance of dissociated or fractionated subunit preparations. Recombinant large subunits were purified to a state of homogeneity and retained reconstitution capacity in the presence of added small subunits. The purified large… CONTINUE READING