Purification of recombinant adeno-associated virus vectors by column chromatography and its performance in vivo.

@article{Gao2000PurificationOR,
  title={Purification of recombinant adeno-associated virus vectors by column chromatography and its performance in vivo.},
  author={Guang-ping Gao and Guang Qu and Mac Burnham and Jialing Huang and Narendra Chirmule and Bhavesh H. Joshi and Qian-chun Yu and James A. Marsh and Cl{\'a}udia Conceiç{\~a}o and James Michael Wilson},
  journal={Human gene therapy},
  year={2000},
  volume={11 15},
  pages={2079-91}
}
Recombinant adeno-associated virus (AAV) holds much promise for human gene therapy. While evidence indicates that AAV mediates long-term gene transfer in several different tissues, difficulty in preparing and purifying this viral vector in large quantities remains a major obstacle for evaluating AAV vectors in clinical trials. The current method of purification, based on sedimentation through cesium chloride, is not scaleable and yields product of insufficient quality. In this article we report… CONTINUE READING

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