Purification of recombinant C-reactive protein mutants.

  title={Purification of recombinant C-reactive protein mutants.},
  author={A. Thirumalai and Sanjay K. Singh and D. Hammond and Toh B. Gang and D. N. Ngwa and Asmita Pathak and A. Agrawal},
  journal={Journal of immunological methods},
C-reactive protein (CRP) is an evolutionarily conserved protein, a component of the innate immune system, and an acute phase protein in humans. In addition to its raised level in blood in inflammatory states, CRP is also localized at sites of inflammation including atherosclerotic lesions, arthritic joints and amyloid plaque deposits. Results of in vivo experiments in animal models of inflammatory diseases indicate that CRP is an anti-pneumococcal, anti-atherosclerotic, anti-arthritic and an… Expand
9 Citations
Complement Activation by C-Reactive Protein Is Critical for Protection of Mice Against Pneumococcal Infection
The hypothesis that complement activation by phosphocholine-complexed CRP is an antipneumococcal effector function was supported and it can be concluded that complementactivation by phospholipid complex is indeed essential for CRP-mediated protection of mice against pneumococcal infection. Expand
Treatment of Pneumococcal Infection by Using Engineered Human C-Reactive Protein in a Mouse Model
It is suggested that pre-modified CRP, such as E-CRP, is therapeutically beneficial to decrease bacteremia in pneumococcal infection and a model for the mechanism of action of CRP is proposed. Expand
Monomeric C-reactive protein regulates fibronectin mediated monocyte adhesion.
In vitro and in vivo evidence is provided that mCRP formed by local dissociation from circulating CRP is the major isoform that interacts with FN and regulates FN-mediated monocyte adhesion, which is involved in the pro-inflammatory process. Expand
Hydroxycholesterol binds and enhances the anti-viral activities of zebrafish monomeric c-reactive protein isoforms
Novel preferential bindings of 25-hydroxycholesterol to CRP1-7 compared with other lipids are described and the antiviral effects of both 25HOCh and CRP 1-7 against spring viremia carp virus infection in zebrafish are explored. Expand
25-Hydroxyl-cholesterol Binds and Enhance Anti-viral Effects of Zebrafish Monomeric C-reactive Proteins
In this study, preferential 25-hydroxycholesterol binding to zebrafish CRP1-7 compared to other lipids were predicted by in silico docking and confirmed by solid-phase binding-assays. Expand
Hyper-acidic fusion minipeptides escort the intrinsic antioxidative ability of the pattern recognition receptor CRP in non-animal organisms
A new insight is provided for understanding the etiology of CRP-involved inflammations and diseases, and a potential is endorsed for CRP biotechnological applications in developing new pharmaceutical therapies and improving plant oxidative resistance is endorsed. Expand
Quantum Dot Based Nano-Biosensors for Detection of Circulating Cell Free miRNAs in Lung Carcinogenesis: From Biology to Clinical Translation
The present review illustrates the importance of circulating miRNAs in lung cancer diagnosis and highlights the translational prospects of developing QD-based nano-biosensor for rapid early disease detection. Expand
Vías de inmovilización de biomoléculas sobre SU-8 y otras superficies poliméricas para su utilización en biosensado óptico sin marcaje
Agradecer a la financiacion de esta investigacion por Garantia Juvenil, FEDER y los proyectos del MINECO CTQ / 2016/75749‐R y TEC / 2017/84846‐R. Ademas, fue apoyado por el Programa Horizonte 2020Expand


Human C-reactive protein: expression, structure, and function.
The weight of the evidence is that CRP like other acute-phase proteins is a component first line of innate host defense and appears to be host defense against bacterial pathogens and clearance of apoptotic and necrotic cells. Expand
Exposing a Hidden Functional Site of C-reactive Protein by Site-directed Mutagenesis*
The E42Q CRP mutant provides a tool to investigate the functions of CRP in defined animal models of inflammatory diseases including atherosclerosis because wild-type CRP requires acidic pH to bind to deposited, conformationally altered proteins, including ox-LDL, and available animal models may not have sufficient acidosis or other possible modifiers of the pentameric structure ofCRP at the sites of inflammation. Expand
Engineered C-reactive protein with improved protective action against pneumococcal infection
The anti-pneumococcal function of native C-reactive protein (CRP) involves the binding of CRP to phosphocholine molecules present on Streptococcus pneumoniae and subsequent activation of theExpand
C-reactive Protein*
This review will largely focus on the current understanding of the structure of CRP, its ligands, the effector molecules with which it interacts, and its apparent functions. Expand
Role of the Property of C-Reactive Protein to Activate the Classical Pathway of Complement in Protecting Mice from Pneumococcal Infection1
The mouse is an unfit animal for exploring CRP-mediated activation of the classical pathway of complement pathway, and that the characteristic of CRP to activate the classical complement pathway has no role in protecting mice from infection. Expand
The protective function of human C-reactive protein in mouse models of Streptococcus pneumoniae infection.
Human C-reactive protein, injected intravenously into mice or produced inside mice by a human transgene, protects mice from death following administration of lethal numbers of Streptococcus pneumoniae, suggesting that CRP works prophylactically but not as a treatment for infection. Expand
Recognition Functions of Pentameric C-Reactive Protein in Cardiovascular Disease
C-reactive protein (CRP) performs two recognition functions that are relevant to cardiovascular disease. First, in its native pentameric conformation, CRP recognizes molecules and cells with exposedExpand
Solubilization and purification of recombinant modified C-reactive protein from inclusion bodies using reversible anhydride modification
A method using anhydride reagents to effectively solubilize rmCRP and allow for chromatographic purification in high yield and free of contaminating endotoxin is described. Expand
Human C-reactive protein is protective against fatal Streptococcus pneumoniae infection in transgenic mice.
This study provides the first unequivocal evidence that CRP plays an important role in vivo in host defense against pneumococcal infections, and shows that sex hormones can affect expression of the human CRP transgene in mice. Expand
Atherosclerosis-related functions of C-reactive protein.
It is possible that an inflammatory environment, such as those characterized by acidic pH, is needed for efficient interaction between CRP and atherogenic LDL during the development of atherosclerosis and to observe the possible atheroprotective function of CRP in animal models. Expand