Purification of rat heart and rat liver citrate synthases. Physical, kinetic, and immunological studies.

Abstract

The citrate synthases from rat heart and rat liver have been purified. These homogeneous proteins have a molecular weight of about 1 x lo5 and are composed of 2 apparently identical subunits. Kinetic constants for acetyl coenzyme A and oxaloacetate and the Ki for ATP are the same for both enzymes. Immunological studies using antibody to the rat heart enzyme also indicate that the enzymes are identical.

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@article{Moriyama1971PurificationOR, title={Purification of rat heart and rat liver citrate synthases. Physical, kinetic, and immunological studies.}, author={T Moriyama and Paul A. Srere}, journal={The Journal of biological chemistry}, year={1971}, volume={246 10}, pages={3217-23} }