Purification of properties of dihydroorotase, a zinc-containing metalloenzyme in Clostridium oroticum.


Dihydroorotase +4,5-L-dihydro-orotate amidohydrolase [EC]), which catalyzes the reversible cyclization of N-carbamyl-L-aspartate to L-dihydroorotate, has been purified from orotate-grown Clostridium oroticum. The enzyme is homogeneous when subjected to polyacrylamide gel electrophoresis and is stable at pH 7.6 in 0.3 M NaCl containing 10 muM ZnSO4… (More)


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