Purification of proctolin-binding proteins from the foregut of the insect Blaberus craniifer.

@article{Mazzocco2000PurificationOP,
  title={Purification of proctolin-binding proteins from the foregut of the insect Blaberus craniifer.},
  author={Claire Mazzocco and Jacques Puiroux},
  journal={European journal of biochemistry},
  year={2000},
  volume={267 8},
  pages={2252-9}
}
A membrane protein that specifically binds the insect neuropeptide proctolin was purified using standard chromatography from cockroach foregut membranes. Proctolin-binding sites were efficiently solubilized with either the nonionic detergent digitonin or the zwitterionic detergent Chaps, as indicated by the specific binding of 3H-proctolin to solubilized… CONTINUE READING