Purification of polyclonal antibodies from Cohn fraction II + III, skim milk, and whey by affinity chromatography using a hexamer peptide ligand.

@article{Menegatti2012PurificationOP,
  title={Purification of polyclonal antibodies from Cohn fraction II + III, skim milk, and whey by affinity chromatography using a hexamer peptide ligand.},
  author={Stefano Menegatti and Amith Dattatray Naik and Patrick V. Gurgel and Ruben Guillermo Carbonell},
  journal={Journal of separation science},
  year={2012},
  volume={35 22},
  pages={3139-48}
}
HWRGWV, a peptide that binds specifically to the Fc fragment of human immunoglobulin G (IgG), was used for the purification of IgG from Cohn fraction II + III of human plasma and from bovine skim milk and whey. The concentration of sodium chloride and sodium caprylate in the binding buffer as well as the pH of the elution buffer were optimized to achieve… CONTINUE READING