Purification of pig renin.

  title={Purification of pig renin.},
  author={W. Stanley Peart and A M Lloyd and Gary Thatcher and Anthony F Lever and Nadine N. Payne and Neil J. Stone},
  journal={The Biochemical journal},
  volume={99 3},
1. A new method of purification of renin is described. This method employs the following procedures: ethanol precipitation; saline extraction; precipitation of renin with 40% ammonium sulphate; precipitation of impurities with 3% ammonium sulphate at pH2.5; chromatography on DEAE-cellulose and CM-Sephadex; gel filtration on Sephadex G-100 (both normal and superfine grade); finally, starch-gel electrophoresis. 2. The final renin preparation had a specific activity 10(4) times that of the initial… CONTINUE READING


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