Purification of phosphodiesterase from Bothrops atrox venom, with special consideration of the elimination of monophosphatases.

  • W BJORK
  • Published 1963 in The Journal of biological chemistry
In fragments of nucleic acids bearing 3’-monophosphoryl groups, both terminals may be determined by degrading the fragment with a massive dose of a single enzyme, venom phosphodiesterase (l-5). To be reliable, this method requires a preparation of phosphodiesterase which is both highly potent and free from contaminating interfering enzymes. The most… (More)