Purification of peptidyl-tRNA on benzoylated DEAE-cellulose columns.

  title={Purification of peptidyl-tRNA on benzoylated DEAE-cellulose columns.},
  author={Dan Eilat and Ehud Ziv and Y Lapidot},
  journal={Nucleic acids research},
  volume={2 12},
The o-nitrophenylsulfenyl group, and amino protecting group in the chemical synthesis of peptidyl-tRNA was used to attach newly synthesized peptidyl-tRNA to a benzoylated DEAE-cellulose column. This facilitated the isolation of a highly purified specific tRNA with a well defined peptide chain. 

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Abbreviations used: B.D. cellulose, benzoylated DEAE-cellulose; NFS, o-nitrophenylsulfenyl; NPS-Ala-OSu, IMiydroxysuccinimide ester of NPS-alanine; DMS0, dimethylsulfoxide; aa-tRNA, aminoacyl-tRNA

H. Rahamimoff, E. Baksht, Y. Lapidot, N. DeGroot
Eur. J. Biochem • 1972

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