Purification of peptides: an efficient procedure for the separation of peptides from amino acids and salt.

@article{Bhlen1980PurificationOP,
  title={Purification of peptides: an efficient procedure for the separation of peptides from amino acids and salt.},
  author={Peter B{\"o}hlen and F Echeverr{\'i}a Castillo and Nicholas Ling and Roger Guillemin},
  journal={International journal of peptide and protein research},
  year={1980},
  volume={16 4},
  pages={306-10}
}
A novel method for the separation of peptides from amino acids and salts has been developed. Separation is achieved by passage of the sample through a column of octadecasilyl-silica (ODS). Peptides, but not amino acids and salts, are strongly retained by ODS in an aqueous mobile phase and can be recovered by elution with an aqueous-alcoholic mobile phase. The method is applicable to most peptides and is fast and simple. Moreover, it is characterized by good recovery yields and high sample… CONTINUE READING