Purification of neurocatin, a neuroregulatory factor from brain.

  title={Purification of neurocatin, a neuroregulatory factor from brain.},
  author={Daniel K. Yee and Peter Pastuszko and Anna Pastuszko and David F Wilson},
  journal={Neuroscience letters},
  volume={114 2},
A neuroregulatory factor (neurocatin) has been isolated from bovine brain. Neurocatin is a powerful affector of catecholamine metabolism in synaptosomes isolated from rat brain, causing increased formation of norepinephrine (NE) and decreased formation of 3,4-dihydroxyphenylacetic acid (DOPAC). The ratio NE/DOPAC in synaptosomes has been used to measure the neurocatin content of samples during isolation. Neurocatin has now been purified to a single chromatographic peak by high-resolution HPLC… CONTINUE READING