Purification of mouse brain (Na+ + K+)-ATPase catalytic unit, characterization of antiserum, and immunocytochemical localization in cerebellum, choroid plexus, and kidney.

@article{Siegel1984PurificationOM,
  title={Purification of mouse brain (Na+ + K+)-ATPase catalytic unit, characterization of antiserum, and immunocytochemical localization in cerebellum, choroid plexus, and kidney.},
  author={G. Siegel and Cecilia Holm and James H. Schreiber and Timothy J. Desmond and Stephen A. Ernst},
  journal={The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society},
  year={1984},
  volume={32 12},
  pages={1309-18}
}
The denatured catalytic polypeptide of mouse brain (Na+ + K+)-adenosine triphosphatase(ATPase) was separated from microsomal membranes on polyacrylamide gels and used as an immunogen. The antiserum, characterized by immunoblots, recognizes the polypeptide corresponding to the catalytic unit in various fractions of mouse brain and cross-reacts with the catalytic unit from lamb kidney, duck salt gland, and electroplax. The same polypeptide in brain and salt gland is recognized by antiserum raised… CONTINUE READING

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