Purification of monomeric prolactin charge isoform from buffalo pituitaries.

  title={Purification of monomeric prolactin charge isoform from buffalo pituitaries.},
  author={Manoj Panchal and Kambadur Muralidhar},
  journal={Preparative biochemistry & biotechnology},
  volume={38 1},
A standard protocol for isolation of buffalo prolactin (buPRL) was modified at the alcohol precipitation step. This modification could separate lower molecular weight prolactin from the higher molecular weight prolactin (PRL). Reloading the prolactin onto a Sephacryl S-200 gel purified the buPRL monomer. The purity of buPRL monomer was confirmed by 15% SDS PAGE. The buPRL monomer was >90% pure. It was characterized by specific anti-buPRL serum in ELISA and Western blot. A native PAGE of the PRL… CONTINUE READING