Purification of membrane proteins.

  title={Purification of membrane proteins.},
  author={Thomas C. Thomas and Mark G. McNamee},
  journal={Methods in enzymology},
As stated at the beginning of this chapter, it is not possible to present a single step-by-step protocol for the purification of all membrane proteins. We have discussed many of the individual techniques employed to purify membrane proteins and the problems associated with their application. A successful purification protocol will require the use of a combination of these techniques. It should be clear from the examples given, however, that affinity chromatography is by far the most useful… CONTINUE READING

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