Purification of mammalian DNA polymerases: DNA polymerase α

  title={Purification of mammalian DNA polymerases: DNA polymerase α},
  author={Teresa S.-F. Wang and William C. Copeland and Lars Rogge and Qun Dong},
Publisher Summary This chapter discusses the purification of mammalian DNA polymerases, DNA polymerase α. DNA polymerase α plays a key role in the initiation and replication of the mammalian chromosome. The production of a panel of monoclonal antibodies against human DNA polymerase α allowed the development of a three-step immunoaffinity purification protocol. The monoclonal antibodies were used as the immunoligand bound to a protein A-Sepharose 4B resin to purify the DNA polymerase α from… CONTINUE READING


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