Purification of major fimbrial proteins of Porphyromonas gingivalis.

@article{Lee1995PurificationOM,
  title={Purification of major fimbrial proteins of Porphyromonas gingivalis.},
  author={J Y Lee and Hakimuddin T. Sojar and Atsuo Amano and Robert J. Genco},
  journal={Protein expression and purification},
  year={1995},
  volume={6 4},
  pages={496-500}
}
Binding of Porphyromonas gingivalis to pellicle-coated teeth is mediated to a large extent by fimbrillin, a major structural subunit of fimbriae. A simple and efficient method for purifying the major fimbrial proteins from various strains of P. gingivalis was developed. The sonic extracts of crude fimbriae were prepared from P. gingivalis strains 2561, A7A1-28, EM-3, and 9-14K-1, which are representatives of different fimbrial groups. The crude fimbriae were precipitated from the extracts with… CONTINUE READING
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