Purification of l-α-Glycerylphosphorylcholine from the Enzyme Reaction Solutions by Resin Column Chromatography

@article{Zhang2012PurificationOL,
  title={Purification of l-α-Glycerylphosphorylcholine from the Enzyme Reaction Solutions by Resin Column Chromatography},
  author={Kangyi Zhang and Xingguo Wang and Yuanfa Liu},
  journal={Journal of the American Oil Chemists' Society},
  year={2012},
  volume={89},
  pages={1155-1163}
}
Colorless l-α-glycerylphosphorylcholine (l-α-GPC) was obtained at 98.8% purity, and with a specific rotation of −2.5° via a four-step procedure. l-α-GPC was first produced by phospholipase A1 hydrolysis of phosphatidylcholine in an aqueous medium. Almost all the impurities were then removed using two successive D001 cation and D301-111 anion exchange resin column chromatography procedures, followed by decoloration with active carbon to remove minor pigments and remaining impurities. D001 resin… CONTINUE READING