Purification of isoleucyl-tRNA synthetase from Methanobacterium thermoautotrophicum by pseudomonic acid affinity chromatography.

@article{Rechsteiner1989PurificationOI,
  title={Purification of isoleucyl-tRNA synthetase from Methanobacterium thermoautotrophicum by pseudomonic acid affinity chromatography.},
  author={Thomas Rechsteiner and Thomas Leisinger},
  journal={European journal of biochemistry},
  year={1989},
  volume={181 1},
  pages={
          41-6
        }
}
The isoleucyl-tRNA synthetase of the archaebacterium Methanobacterium thermoautotrophicum was purified 1500-fold to electrophoretic homogeneity by a procedure based on affinity chromatography on Sepharose-bound pseudomonic acid, a strong competitive inhibitor of this enzyme. The purified enzyme is a monomer with a molecular mass of 120 kDa. In this respect and in its Km values for the PPi-ATP exchange, and aminoacylation reactions, it resembles the isoleucyl-tRNA synthetases from eubacterial… Expand
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References

SHOWING 1-10 OF 26 REFERENCES
Phenylalanyl-tRNA synthetase from the archaebacterium Methanosarcina barkeri.
TLDR
Heterologous aminoacylation of tRNA with high selectivity for Archaebacterial tRNA and substrate properties of ATP analogues reveals a unique pattern, reflecting the supposed genealogical difference between the urkingdoms of archaebacteria, eubacteria, and eukaryotes. Expand
Isolation and properties of isoleucyl-tRNA synthetase from Escherichia coli MRE 600.
A method is described for the isolation of isoleucyl-tRNA synthetase from Escherichia coli MRE 600 involving gel filtration, ion-exchange chromatography on DEAE-Sephadex and chromatography onExpand
Isoleucyl-transfer ribonucleic acid synthetase from Bacillus stearothermophilus. I. Properties of the enzyme.
TLDR
The properties of the purified isoleucyl-tRNA synthetase from Bacillus stearothermophilus are reported on and a clearcut requirement of these ions for the formation and/or stabilization of the tRNA · enzyme complex has been demonstrated. Expand
Interaction of pseudomonic acid A with Escherichia coli B isoleucyl-tRNA synthetase.
TLDR
Sodium pseudomonate was shown to be a powerful competitive inhibitor of Escherichia coli B isoleucyl-tRNA synthetase (Ile-t RNAs) and has no effect on the transfer of [14C]isoleucine from the enzyme . Expand
Purification of isoleucyl transfer ribonucleic acid synthetase by affinity chromatography on blue dextran—Sepharose
TLDR
The purification of IRS of Escherichia coli (EC 6.1.5) is reported to near-homogeneity from crude cellular extracts by chromatography on two sequential blue dextranSepharose columns. Expand
Mutation to pseudomonic acid resistance of Methanobacterium thermoautotrophicum leads to an altered isoleucyl-tRNA synthetase
Growth of Methanobacterium thermoautotrophicum strain Marburg was strongly inhibited by 2 μg/ml of pseudomonic acid. This inhibition was relieved by 10 mM l-isoleucine. The susceptibility of aExpand
Purification and properties of isoleucyl ribonucleic acid synthetase from Escherichia coli.
TLDR
The enzyme is protected from heat inactivation and tryptic digestion by the presence of adenosine triphosphate and Mg++ plus saturating amounts of isoleucine or valine. Expand
Purification and characterization of the isoleucyl-tRNA synthetase component from the high molecular weight complex of sheep liver: a hydrophobic metalloprotein.
TLDR
It is suggested that the assembly of the amino-acyl-tRNA synthetase complex is mediated by hydrophobic domains present in these enzymes. Expand
Kinetics of aminoacyl-tRNA synthetases catalyzed ATP-PPi exchange.
Publisher Summary Most of the aminoacyl-tRNA synthetases catalyze an amino acid-dependent triphosphate-pyrophosphate (ATP-PP i) exchange in the absence of their cognate tRNA's. Unlike mostExpand
Mechanism of aminoacylation of tRNA. Proof of the aminoacyl adenylate pathway for the isoleucyl- and tyrosyl-tRNA synthetases from Escherichia coli K12.
TLDR
Observations show that the formation of isoleucyl-tRNA catalyzed by the isoleurcyl- tRNA synthetase involves the initial rapid formation of an isoleUcyl adenylate complex followed by the slow, rate-determining, transfer of the isolucyl moiety to tRNA. Expand
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