Purification of isoleucyl-tRNA synthetase from Methanobacterium thermoautotrophicum by pseudomonic acid affinity chromatography.

  title={Purification of isoleucyl-tRNA synthetase from Methanobacterium thermoautotrophicum by pseudomonic acid affinity chromatography.},
  author={Thomas Rechsteiner and Thomas Leisinger},
  journal={European journal of biochemistry},
  volume={181 1},
The isoleucyl-tRNA synthetase of the archaebacterium Methanobacterium thermoautotrophicum was purified 1500-fold to electrophoretic homogeneity by a procedure based on affinity chromatography on Sepharose-bound pseudomonic acid, a strong competitive inhibitor of this enzyme. The purified enzyme is a monomer with a molecular mass of 120 kDa. In this respect and in its Km values for the PPi-ATP exchange, and aminoacylation reactions, it resembles the isoleucyl-tRNA synthetases from eubacterial… Expand
5 Citations
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Isolation and properties of isoleucyl-tRNA synthetase from Escherichia coli MRE 600.
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Interaction of pseudomonic acid A with Escherichia coli B isoleucyl-tRNA synthetase.
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Purification of isoleucyl transfer ribonucleic acid synthetase by affinity chromatography on blue dextran—Sepharose
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Mutation to pseudomonic acid resistance of Methanobacterium thermoautotrophicum leads to an altered isoleucyl-tRNA synthetase
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The enzyme is protected from heat inactivation and tryptic digestion by the presence of adenosine triphosphate and Mg++ plus saturating amounts of isoleucine or valine. Expand
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Kinetics of aminoacyl-tRNA synthetases catalyzed ATP-PPi exchange.
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