Purification of interferon gamma-interferon gamma receptor complexes by preparative electrophoresis on native gels.

@article{Fountoulakis1993PurificationOI,
  title={Purification of interferon gamma-interferon gamma receptor complexes by preparative electrophoresis on native gels.},
  author={Michael Fountoulakis and E Takacs-di Lorenzo and Jean François Juranville and M Manneberg},
  journal={Analytical biochemistry},
  year={1993},
  volume={208 2},
  pages={270-6}
}
Cytokine-receptor complexes are required for certain studies including crystallization, NMR spectra, and investigation of the biological response mechanism to the cytokine. The purity of the ligand-receptor complex is critical for most of these applications. We investigated the possibility of purifying protein-protein complexes by electrophoresis on native gels. Starting with partially purified mouse and highly purified human proteins, we prepared milligram amounts of interferon gamma… CONTINUE READING

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