Purification of insoluble nitric oxide synthase from rat cerebellum.

  title={Purification of insoluble nitric oxide synthase from rat cerebellum.},
  author={K Hiki and Ryuichi Hattori and Chuichi Kawai and Yoshiki Yui},
  journal={Journal of biochemistry},
  volume={111 5},
Nitric oxide synthase [EC 1.14.23] from the particulate fraction of rat cerebella was purified and characterized. The homogenate of rat cerebella was centrifuged to obtain a pellet, which was washed and incubated with Triton X-100 containing buffer. The enzyme activity appeared in the 100,000 x g supernatant after incubation with the detergent. The solubilized enzyme was then purified by sequential affinity chromatography using adenosine 2',5'-diphosphate agarose and calmodulin Sepharose 4B… CONTINUE READING


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