[Purification of human VLDL apolipoproteins by middle pressure liquid chromatography].


OBJECTIVE To purify human VLDL apolipoproteins by middle-pressure liquid chromatography. METHODS Human VLDLs were isolated by one step density ultracentrifugation. Delipided human VLDL was separated by Sephacryl S-200 molecular sieve chromatography. ApoE was purified by heparin Sepharose CL-6B affinity chromatography. ApoC I ,C II and C III were purified from apoC. fraction by DEAE-Sephacel ion exchange chromatography: RESULTS Purified apoE, apoC I, apoC II and apoC III were obtained. SDS-PAGE and immunodiffusion tests indicated the isolated proteins were pure. CONCLUSION We have established a purification procedure for human VLDL apolipoproteins with highly efficiency and simplicity by MPLC.

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@article{Xin2007PurificationOH, title={[Purification of human VLDL apolipoproteins by middle pressure liquid chromatography].}, author={Yan-fei Xin and Yun Duan and Zu-Yue Deng and Zu-hui Zhang and Bing-wen Liu}, journal={Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition}, year={2007}, volume={38 2}, pages={328-30, 333} }