Purification of glycomacropeptide from non-dialyzable fraction of sweet whey by anion-exchange chromatography

@inproceedings{Nakano1999PurificationOG,
  title={Purification of glycomacropeptide from non-dialyzable fraction of sweet whey by anion-exchange chromatography},
  author={Takuo Nakano and Lech Ozimek},
  year={1999}
}
Glycomacropeptide (GMP) was purified from non-dialyzable fraction of sweet whey by anion-exchange chromatography on DEAE-Sephacel at two pHs 6.4 and 3.0. Chromatography at pH 3.0 (but not pH 6.4) gave a GMP fraction of high purity with its yield (1 g from every litre of whey) being approximately 100 times higher than that shown in the previous report. It was concluded that DEAE-Sephacel chromatography at pH 3.0 is a simple useful method to separate GMP from most whey proteins. It may be… CONTINUE READING