Purification of functional T lymphocytes from splenocytes of the beluga whales (Delphinapterus leucas).

@article{Bernier2000PurificationOF,
  title={Purification of functional T lymphocytes from splenocytes of the beluga whales (Delphinapterus leucas).},
  author={Jacques Bernier and Sylvain De Guise and Daniel Martineau and Pierre B{\'e}land and M J Beaudet and Michel Fournier},
  journal={Developmental and comparative immunology},
  year={2000},
  volume={24 6-7},
  pages={653-62}
}
In an effort to gain knowledge on immune functions in beluga whales, Delphinapterus leucas, we have used two physical methods for the purification of T lymphocytes of spleen cells. Isolation by sheep red blood cells (SRBC) rosetting and by adherence on nylon wool columns were tested. SRBC-rosetting gave unreliable results in obtaining purified T cells. Therefore, the purification of T cells was done using nylon wool columns. Less than 3% of the IgM(+) B cells remained in effluent populations… CONTINUE READING